产品概述：Purified recombinant protein of Mouse ubiquitin-conjugating enzyme E2O (Ube2o), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

蛋白长度：A DNA sequence from Mouse cDNA ORF Clone, [GM49698V], encoding Mouse full-length Ube2o.

表达系统：HEK293T

种属：Mouse

标签类型：C-MYC/DDK

分子量：141.3 kDa

通用名：Ube2o

总结：E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.[UniProtKB/Swiss-Prot Function]

形式：Liquid

浓度：>50 ug/mL as determined by microplate BCA method

纯度：> 80% as determined by SDS-PAGE and Coomassie blue staining

储存溶液：25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

别名：9630022H21; B230113M03Rik; E2-230K; mKIAA1734